Immunochemical Assays for Determination of Organophosphorus Pesticides in Milk

Determination of Twenty Organophosphorus Pesticides in Wheat Samples from Different Regions of Iran

Background: Organophosphorus pesticides are widely used in agriculture, homes, gardens, and veterinary practices. Extensive application of pesticides in agriculture often results in residues of these compounds being absorbed into the foods, including wheat. The aim of this study was to evaluate the residue levels of 20 organophosphorus pesticides in wheat samples collected from different region...

Development of Phage Immuno-Loop-Mediated Isothermal Amplification Assays for Organophosphorus Pesticides in Agro-products

Two immuno-loop-mediated isothermal amplification assays (iLAMP) were developed by using a phage-borne peptide that was isolated from a cyclic eight-peptide phage library. One assay was used to screen eight organophosphorus (OP) pesticides with limits of detection (LOD) between 2 and 128 ng mL(-1). The iLAMP consisted of the competitive immuno-reaction coupled to the LAMP reaction for detection...

Multiresidue determination of organophosphorus pesticides in corn oil using solid-phase extraction with lanthanum silicate sorbent and gas chromatography

Phosalone, malathion and diazinon were analyzed in corn oil using solid phase extraction (SPE)with lanthanum silicate as a new solid sorbent followed by gas chromatography with nitrogenphosphorus detection (GC-NPD). The efficiency of different eluents (methanol, dichloromethane,ethyl acetate, dichloromethane — ethyl acetate (1:1) and ethyl acetate — methanol (1:1)) werecompared for elution of o...

Organophosphorus hydrolase-based assay for organophosphate pesticides

We report a rapid and versatile organophosphorus hydrolase (OPH)-based method for measurement of organophosphates. This assay is based on a substrate-dependent change in pH at the local vicinity of the enzyme. The pH change is monitored using fluorescein isothiocyanate (FITC), which is covalently immobilized to the enzyme. This method employs the use of poly(methyl methacrylate) beads to which ...

Recent Trends in the Development of Electrochemical Biosensors for Organophosphorus Pesticides Determination